pcdna3 1 ha hhoil 1l (Addgene inc)
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pcdna3 1 ha hhoil 1l
Pcdna3 1 Ha Hhoil 1l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+ha+hhoil+1l/pHAGE-EGFR-P699L+(Plasmid+%23116286)/pm40944911-277-279-275
Average 93 stars, based on 1 article reviews
Pcdna3 1 Ha Hhoil 1l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+ha+hhoil+1l/pHAGE-EGFR-P699L+(Plasmid+%23116286)/pm40944911-277-279-275
Average 93 stars, based on 1 article reviews
pcdna3 1 ha hhoil 1l - by Bioz Stars,
2026-10
93/100 stars
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Recombinant:Article Title: Pathogenic phosphorylation of linear ubiquitin machinery causes inflammasome sensor degradation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human RBCK1 MedChemExpress Cat# HY-P702076 Recombinant human NLRP3 (GST-tagged) MedChemExpress Cat# HY-P701027 Critical commercial assays Phanta Max Super-Fidelity DNA Polymerase Vazyme Cat# P505 Fast Mutagenesis Kit V2 Vazyme Cat# C214 LDH Cytotoxicity Assay Kit Beyotime Cat# C0016 BCA Protein Assay Kit Beyotime Cat# P0010 Total RNA Extraction Kit GDSBio Cat# R1061 Hifair II 1st Strand cDNA Synthesis SuperMix for qPCR Yeasen Cat# 11123ES10 Hieff qPCR SYBR Green Master Mix Yeasen Cat# 11200ES08 Malachite Green Phosphate Detection Kit Cell Signaling Technology Cat# 12776 Human IL-1β ELISA Kit RayBiotech Cat# ELH-IL1b Mouse IL-1β ELISA Kit RayBiotech Cat# ELM-IL1b Mouse IL-6 ELISA Kit RayBiotech Cat# ELM-IL6 Mouse TNF-α ELISA Kit RayBiotech Cat# ELM-TNFa Mouse IFN-γ ELISA Kit Cloud-Clone Corp. 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Shao N/A Plasmid: pGBKT7-PknG Wang et al. 33 N/A Plasmid: Real-time Polymerase Chain Reaction:Article Title: Pathogenic phosphorylation of linear ubiquitin machinery causes inflammasome sensor degradation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human RBCK1 MedChemExpress Cat# HY-P702076 Recombinant human NLRP3 (GST-tagged) MedChemExpress Cat# HY-P701027 Critical commercial assays Phanta Max Super-Fidelity DNA Polymerase Vazyme Cat# P505 Fast Mutagenesis Kit V2 Vazyme Cat# C214 LDH Cytotoxicity Assay Kit Beyotime Cat# C0016 BCA Protein Assay Kit Beyotime Cat# P0010 Total RNA Extraction Kit GDSBio Cat# R1061 Hifair II 1st Strand cDNA Synthesis SuperMix for qPCR Yeasen Cat# 11123ES10 Hieff qPCR SYBR Green Master Mix Yeasen Cat# 11200ES08 Malachite Green Phosphate Detection Kit Cell Signaling Technology Cat# 12776 Human IL-1β ELISA Kit RayBiotech Cat# ELH-IL1b Mouse IL-1β ELISA Kit RayBiotech Cat# ELM-IL1b Mouse IL-6 ELISA Kit RayBiotech Cat# ELM-IL6 Mouse TNF-α ELISA Kit RayBiotech Cat# ELM-TNFa Mouse IFN-γ ELISA Kit Cloud-Clone Corp. Cat# SEA049Mu Experimental models: Cell lines HEK293T cells ATCC Cat# CRL-3216; RRID: CVCL_0063 iBMDMs Dr. F. Shao N/A iBMDMs (NLRP3-knockout) This study N/A iBMDMs (AIM2-knockout) This study N/A iBMDMs (HOIL-1L-knockout) This study N/A Experimental models: Organisms/strains Mouse: C57BL/6 Beijing Vital River Laboratory Animal Technology Cat# 213 Mouse: C57BL/6 (Nlrp3 − /− ) Dr. F. Shao N/A Mouse: C57BL/6 (Gsdmd − /− ) Dr. F. Shao N/A Oligonucleotides See Table S4 for primer sequences N/A N/A Recombinant DNA Plasmid: pEGFP-N1 Clontech Cat# 6085-1 Plasmid: pEGFP-N1-ASC This study N/A Plasmid: pCS2-Flag-NLRP3 Dr. F. Shao N/A Plasmid: pCS2-Myc-AIM2 Dr. F. Shao N/A Plasmid: pCS2-Myc-ASC Dr. F. Shao N/A Plasmid: pCS2-Myc-IL-1β Dr. F. Shao N/A Plasmid: pCS2-Flag-caspase-1 Dr. F. Shao N/A Plasmid: pGADT7 Clontech Cat# 630442 Plasmid: pGADT7-HOIL-1L This study N/A Plasmid: pGADT7-TAK1 Dr. F. Shao N/A Plasmid: pGBKT7 Clontech Cat# 630443 Plasmid: pGBKT7-TAB2 Dr. F. Shao N/A Plasmid: pGBKT7-PknG Wang et al. 33 N/A Plasmid: SYBR Green Assay:Article Title: Pathogenic phosphorylation of linear ubiquitin machinery causes inflammasome sensor degradation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human RBCK1 MedChemExpress Cat# HY-P702076 Recombinant human NLRP3 (GST-tagged) MedChemExpress Cat# HY-P701027 Critical commercial assays Phanta Max Super-Fidelity DNA Polymerase Vazyme Cat# P505 Fast Mutagenesis Kit V2 Vazyme Cat# C214 LDH Cytotoxicity Assay Kit Beyotime Cat# C0016 BCA Protein Assay Kit Beyotime Cat# P0010 Total RNA Extraction Kit GDSBio Cat# R1061 Hifair II 1st Strand cDNA Synthesis SuperMix for qPCR Yeasen Cat# 11123ES10 Hieff qPCR SYBR Green Master Mix Yeasen Cat# 11200ES08 Malachite Green Phosphate Detection Kit Cell Signaling Technology Cat# 12776 Human IL-1β ELISA Kit RayBiotech Cat# ELH-IL1b Mouse IL-1β ELISA Kit RayBiotech Cat# ELM-IL1b Mouse IL-6 ELISA Kit RayBiotech Cat# ELM-IL6 Mouse TNF-α ELISA Kit RayBiotech Cat# ELM-TNFa Mouse IFN-γ ELISA Kit Cloud-Clone Corp. Cat# SEA049Mu Experimental models: Cell lines HEK293T cells ATCC Cat# CRL-3216; RRID: CVCL_0063 iBMDMs Dr. F. 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Shao N/A iBMDMs (NLRP3-knockout) This study N/A iBMDMs (AIM2-knockout) This study N/A iBMDMs (HOIL-1L-knockout) This study N/A Experimental models: Organisms/strains Mouse: C57BL/6 Beijing Vital River Laboratory Animal Technology Cat# 213 Mouse: C57BL/6 (Nlrp3 − /− ) Dr. F. Shao N/A Mouse: C57BL/6 (Gsdmd − /− ) Dr. F. Shao N/A Oligonucleotides See Table S4 for primer sequences N/A N/A Recombinant DNA Plasmid: pEGFP-N1 Clontech Cat# 6085-1 Plasmid: pEGFP-N1-ASC This study N/A Plasmid: pCS2-Flag-NLRP3 Dr. F. Shao N/A Plasmid: pCS2-Myc-AIM2 Dr. F. Shao N/A Plasmid: pCS2-Myc-ASC Dr. F. Shao N/A Plasmid: pCS2-Myc-IL-1β Dr. F. Shao N/A Plasmid: pCS2-Flag-caspase-1 Dr. F. Shao N/A Plasmid: pGADT7 Clontech Cat# 630442 Plasmid: pGADT7-HOIL-1L This study N/A Plasmid: pGADT7-TAK1 Dr. F. Shao N/A Plasmid: pGBKT7 Clontech Cat# 630443 Plasmid: pGBKT7-TAB2 Dr. F. Shao N/A Plasmid: pGBKT7-PknG Wang et al. 33 N/A Plasmid: Plasmid Preparation:Article Title: Pathogenic phosphorylation of linear ubiquitin machinery causes inflammasome sensor degradation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human RBCK1 MedChemExpress Cat# HY-P702076 Recombinant human NLRP3 (GST-tagged) MedChemExpress Cat# HY-P701027 Critical commercial assays Phanta Max Super-Fidelity DNA Polymerase Vazyme Cat# P505 Fast Mutagenesis Kit V2 Vazyme Cat# C214 LDH Cytotoxicity Assay Kit Beyotime Cat# C0016 BCA Protein Assay Kit Beyotime Cat# P0010 Total RNA Extraction Kit GDSBio Cat# R1061 Hifair II 1st Strand cDNA Synthesis SuperMix for qPCR Yeasen Cat# 11123ES10 Hieff qPCR SYBR Green Master Mix Yeasen Cat# 11200ES08 Malachite Green Phosphate Detection Kit Cell Signaling Technology Cat# 12776 Human IL-1β ELISA Kit RayBiotech Cat# ELH-IL1b Mouse IL-1β ELISA Kit RayBiotech Cat# ELM-IL1b Mouse IL-6 ELISA Kit RayBiotech Cat# ELM-IL6 Mouse TNF-α ELISA Kit RayBiotech Cat# ELM-TNFa Mouse IFN-γ ELISA Kit Cloud-Clone Corp. Cat# SEA049Mu Experimental models: Cell lines HEK293T cells ATCC Cat# CRL-3216; RRID: CVCL_0063 iBMDMs Dr. F. Shao N/A iBMDMs (NLRP3-knockout) This study N/A iBMDMs (AIM2-knockout) This study N/A iBMDMs (HOIL-1L-knockout) This study N/A Experimental models: Organisms/strains Mouse: C57BL/6 Beijing Vital River Laboratory Animal Technology Cat# 213 Mouse: C57BL/6 (Nlrp3 − /− ) Dr. F. Shao N/A Mouse: C57BL/6 (Gsdmd − /− ) Dr. F. Shao N/A Oligonucleotides See Table S4 for primer sequences N/A N/A Recombinant DNA Plasmid: pEGFP-N1 Clontech Cat# 6085-1 Plasmid: pEGFP-N1-ASC This study N/A Plasmid: pCS2-Flag-NLRP3 Dr. F. Shao N/A Plasmid: pCS2-Myc-AIM2 Dr. F. Shao N/A Plasmid: pCS2-Myc-ASC Dr. F. Shao N/A Plasmid: pCS2-Myc-IL-1β Dr. F. Shao N/A Plasmid: pCS2-Flag-caspase-1 Dr. F. Shao N/A Plasmid: pGADT7 Clontech Cat# 630442 Plasmid: pGADT7-HOIL-1L This study N/A Plasmid: pGADT7-TAK1 Dr. F. Shao N/A Plasmid: pGBKT7 Clontech Cat# 630443 Plasmid: pGBKT7-TAB2 Dr. F. Shao N/A Plasmid: pGBKT7-PknG Wang et al. 33 N/A Plasmid: |